Fahimeh Geraminezhad Mohammad Roostaei-Ali Mehr


Experiment was conducted to determine effect of Zataria multiflora boiss essential oil on stored spermatozoa. Semen collection was performed by using 15 mature roosters twice a week at four times. In each session, ejaculates were pooled and split into seven parts. The amounts of 0 (EO0), 50 (EO50), 100 (EO100), 200 (EO200), 400 (EO400), 600 (EO600) and 1000 (EO1000) ng/ml Zataria multiflora boiss essential oil were added to each part. Samples were chilled to 4°C and maintained for 72 h. Sperm assessment was performed at 0, 24, 48 and 72 h. Lipid peroxidation was evaluated after 48 h. Results showed that there was no interaction between Zataria multiflora essential oil and incubation time on membrane integrity, sperm motility and viability (p > 0.05). The highest sperm progressive motility (80.43%), viability (86.31%) and functional membrane integrity (85.81%) was observed in EO200 (p < 0.05). The lowest sperm motility (61.31%) and viability (73.31%) was observed in EO1000 (p < 0.05). The concentrations of malondialdehyde was lowest in EO200 (0.17 nM/ml, p < 0.05). Therefore, addition of 200 ng/ml Zataria multiflora boiss essential oil to semen improved longevity of rooster spermatozoa at 4°C.

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Asher, G.W., Berg, D.K., Evans, G. (2000). Storage of semen and artificial insemination in deer. Animal Reparation Science, 62 (1-3), 195-211.
Bailey, J.L., Blodeau, J.F., Cormier, N. (2000). Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon minireview. Journal of andrology, 21 (1), 1-7.
Leboeuf, B., Restall, B., Salamon, S. (2000). Production and storage of goat semen for artificial insemination. Animal Reproduction Science, 62 (1-3), 113-141.
Dhama, K., Singh, R.P., Karthik, K., Chakraborty, S., Tiwari, R., Wani, M.Y., Mohan, J. (2014). Artificial insemination in poultry and possible transmission of infectious pathogens: A review. Asian Journal of Animal and Veterinary Advances, 9 (4), 211-228.
Froman, D.P., Feltmann, A.J., Pendarvis, K., Cooksey, A.M., Burgess, S.C., Rhoads, D.D. (2011). Physiology and endocrinology symposium: a proteome-based model for sperm mobility phenotype. Journal of Animal Science, 89 (5), 1330-1337.
Siudzińska, A., Łukaszewicz, E. (2008). Effect of semen extenders and storage time on sperm morphology of four chicken breeds. The Journal of Applied Poultry Research, 17 (1), 101–108,
Surai, P.F., Blesbois, E., Grasseau, I., Chalah, T., Brillard, J.P., Wishart, G.J., Cerolini, S., Sparks, N.H.C. (1998). Fatty acid composition, glutathione peroxidase and superoxide dismutase activity and total antioxidant activity of avian semen. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 120 (3), 527-533.
Fujihara, N., Howarth Jr, B. (1978). Lipid peroxidation in fowl spermatozoa. Poultry Science, 57 (6), 1766-1768.
Ozkan, A., Erdogan, A., Sokmen, M., Tugrulay, S., Unal, O. (2010). Antitumoral and antioxidant effect of essential oils and in vitro antioxidant properties of essential oils and aqueous extracts from Salvia pisidica. Biologia, 65 (6), 990-996.
Tavakoli, J., Miar, S., Zadehzare, M.M., Akbari, H. (2012). Evaluation of effectiveness of herbal medication in cancer care: a review study. Iranian Journal of Cancer Prevention, 5 (3), 144.
Kavoosi, G., Teixeira da Silva, J.A., Saharkhiz, M.J. (2012). Inhibitory effects of Zataria multiflora essential oil and its main components on nitric oxide and hydrogen peroxide production in lipopolysaccharide‐stimulated macrophages. Journal of Pharmacy and Pharmacology, 64 (10), 1491-1500.
Sharififar, F., Moshafi, M.H., Mansouri, S.H., Khodashenas, M., Khoshnoodi, M. (2007). In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control, 18 (7), 800-805.
Roostaei‐Ali Mehr, M., Parisoush, P. (2016). Effect of Different Levels of Silymarin and Caproic Acid on Storage of Ram Semen in Liquid Form. Reproduction in Domestic Animal, 51 (5), 569-574.
Chung, L.Y., Cheung, T.C., Kong, S.K., Fung, K.P., Choy, Y.M., Chan, Z.H., Kwok, T.T. (2001). Induction of apoptosis by green tea catechins in human prostate cancer DU145 cells. Life Sciences, 68 (10), 1207-1214.
Robaszkiewicz, A., Balcerczyk, A., Bartosz, G. (2007). Antioxidative and prooxidative effects of quercetin on A549 cells. Cell Biology International, 31 (10), 1245-1250.
Ziaeirad, H., Roostaei-Ali Mehr, M., Mohammadi, M., 2016. Effect of silymarin on rooster semen during storage at 4°C. Animal Science Research, 26 (3), 1-13.
Büyükbalci, A., El, S.N. (2008). Determination of in vitro antidiabetic effects, antioxidant activities and phenol contents of some herbal teas. Plant Foods for Human Nutrition, 63 (1), 27-33.
Zheng, W., Wang, S.Y. (2001). Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry, 49 (11), 5165-5170.
Cao, G., Cutler, R.G. (1993). High concentrations of antioxidants may not improve defense against oxidative stress. Archives of Gerontology and Geriatrics, 17 (3), 189-201.
During the time of storage, membrane integrity, sperm viability and motility were declined, which was in agreement with study performed by other investigators [20]. Decrease in quality of stored spermatozoa in liquid form related to the accumulation of the toxic products of metabolism [21]
During the time of storage, membrane integrity, sperm viability and motility were declined, which was in agreement with study performed by other investigators [20]. Decrease in quality of stored spermatozoa in liquid form related to the accumulation of the toxic products of metabolism [21]
Bakkali, F., Averbeck, S., Averbeck, D., Zhiri, A., Idaomar, M. (2005). Cytotoxicity and gene induction by some essential oils in the yeast Saccharomyces cerevisiae. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 585 (1-2), 1-13.
Silva, E.C.B., Cajueiro, J.F.P., Silva, S.V., Soares, P.C., Guerra, M.M.P. (2012). Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology, 77 (8), 1722-1726.
Yu, W., Liu, Q., Zhu, S. (2013). Carvacrol protects against acute myocardial infarction of rats via anti-oxidative and anti-apoptotic pathways. Biological and Pharmaceutical Bulletin, 36 (4), 579-584.
Aydın, S., Başaran, A.A., Başaran, N. (2005). Modulating effects of thyme and its major ingredients on oxidative DNA damage in human lymphocytes. Journal of Agricultural and Food Chemistry, 53 (4), 1299-1305.
Hyldgaard, M., Mygind, T., Meyer, R.L. (2012). Essential oils in food preservation: mode of action, synergies, interactions with food matrix components. Frontiers in Microbiology, 3 (12), 1-24.
Buch, J.G., Dikshit, R.K., Mansuri, S.M. (1988). Effect of certain volatile oils on ejaculated human spermatozoa. Indian Journal of Medical Research, 87, 361-363.
Paul, S., Kang, S.C. (2011). In vitro determination of the contraceptive spermicidal activity of essential oil of Trachyspermum ammi (L.) Sprague ex Turrill fruits. New Biotechnology, 28 (6), 684-690.
Chikhoune, A., Stouvenel, L., Iguer-Ouada, M., Hazzit, M., Schmitt, A., Lorès, P., Wolf, J.P., Aissat, K., Auger, J., Vaiman, D., Touré, A. (2015). In-vitro effects of Thymus munbyanus essential oil and thymol on human sperm motility and function. Reproductive BioMedicine Online, 31 (3), 411-420.
Du, G., Li, M., Ma, F., Liang, D. (2009). Antioxidant capacity and the relationship with polyphenol and vitamin C in Actinidia fruits. Food Chemistry, 113 (2), 557-562.
Park, Y.S., Jung, S.T., Kang, S.G., Heo, B.G., Arancibia-Avila, P., Toledo, F., Drzewiecki, J., Namiesnik, J., Gorinstein, S. (2008). Antioxidants and proteins in ethylene-treated kiwifruits. Food Chemistry, 107 (1-2), 640-648.
Buege, J.A., Aust, S.D. (1978). Microsomal lipid peroxidation. Methods in Enzymology, 52, 302-310.
How to Cite
گرمی نژادف., & روستائی علی مهرم. (2018). Effect of Zataria multiflora essential oil on rooster semen during storage at 4°C. Iranian Journal of Veterinary Science and Technology, 10(1), 21-26. https://doi.org/10.22067/veterinary.v10i1.72126
Original Articles